"We determined the complete mtDNA sequence using a series of 28 overlapping PCR-amplified fragments spanning the entire length of the mitochondrial genome.  Both strands of each PCR fragment were sequenced with BigDye terminator cycle sequencing chemistry on an Applied Biosystems model 377 automated nucleotide sequencer.  In addition, several regions of the CRS mtDNA were sequenced independently using a different approach.  In brief, small PCR-amplified mtDNA fragments of ~300 bp were cloned into M13 sequencing vectors and the mtDNA sequence determined using a dideoxy chain termination method.   The two approaches yielded identical results."